One-Step Blue Protein Gel Stain


SKU: 21003 Category:

Product Description

One-Step Blue™ is a ready-to-use protein gel staining solution. It produces fast (5-60 min) protein staining in a single step without fixation or washing. Proteins can be detected by visible blue staining, or by near-infrared fluorescence. In addition to rapid results and simple staining, One-Step Blue™ offers safer handling and disposal compared to Coomassie staining because it is entirely aqueous-based, without hazardous methanol or acetic acid.

One-Step Blue™ has comparable sensitivity as the widely used Coomassie Blue, with a lower limit of detection around 10-20 ng depending on the detection method used. Note that staining intensity varies between proteins. The staining is fully compatible with mass spectrometry and Edman-based sequencing.

Biotium also offers One-Step Lumitein™ Protein Gel Stain, a rapid, easy-to-use, non-toxic red fluorescent protein gel stain for detection using a UV transilluminator or laser gel scanner, that costs significantly less than other fluorescent protein gels stains.


Flyer (PDF): One-Step Protein Stains

Protocol (PDF): PI-21003

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life