Awaiting product image

Phalloidin, Biotin-XX conjugate

$366.03

Clear
SKU: 28 Categories: , ,

Product Description

Phalloidin and phallacidin are collectively called phallotoxins, which are bicyclic peptides isolated from the deadly Amanita phalloides mushroom. Phallotoxins bind specifically to F-actin filaments with high affinity (1). Fluorescently labeled phallotoxins are very useful tool for investigating the distribution of F-actin. Labeled phallotoxins have similar affinity for both large and small filaments, binding in a stoichiometric ratio of about one phalloidin molecule per actin subunit in muscle and non-muscle cells from various species of plants and animals. Phallotoxins shift the F-actin monomer/polymer equilibrium toward the polymer, lowering the critical concentration for polymerization up to 30-fold (3,4). Phallotoxins also stabilize F-actin filaments, inhibiting depolymerization by cytochalasins, potassium iodide and elevated temperatures. Moreover, phallotoxin-labeled actin filaments remain functional; labeled glycerinated muscle fibers still contract, and labeled actin filaments still move on solid-phase myosin substrates (5,6). Fluorescent phallotoxins can also be used to quantify the amount of F-actin in cells (7,8). Biotin-XX phalloidin stains F-actin with nanomolar affinity. The long flexible spacer group XX facilitates interaction between biotin and avidin or streptavidin which can be labeled with a fluorophore or an enzyme for visualization of F-actin. Biotin-XX phalloidin can also be used for detecting F-actin by electron microscopy. The content in each vial (100 U) is sufficient to stain ~100 samples with 200 uL staining volume per sample.

  • White lyophilized solid soluble in methanol
  • Store at -20°C
  • C57H85N13O14S2
  • MW: 1240

1. Wieland, T. in Phallotoxins, Springer-Verlag, New York (1986).
2. J Muscle Res Cell Motil 9, 370 (1988).
3. Methods Enzymol 85, 514 (1982).
4. Eur J Biochem 165, 125 (1987).
5. Nature 326, 805 (1987).
6. Proc Natl Acad Sci USA 83, 6272 (1986).
7. Blood 69, 945 (1987).
8. Anal Biochem 200, 199 (1992).
9. J Cell Biol 105, 1473 (1987).
10. Proc Natl Acad Sci USA 77, 980 (1980).
11. Nature 284, 405 (1980).
12. CRC Crit Rev Biochem 5, 185 (1978).
13. J Cell Biol 106, 1229 (1988).
14. J Cell Biol 103, 265a (1986).
15. Eur J Cell Biol 24, 176 (1981).
16. Proc Natl Acad Sci USA 74, 5613 (1977).

MSDS (PDF): MSDS 00028
Protocol (PDF): phalloidin

100 U

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life

Related products