Phalloidin, Biotin-XX conjugate



Product Description

Phalloidin and phallacidin are collectively called phallotoxins, which are bicyclic peptides isolated from the deadly Amanita phalloides mushroom. Phallotoxins bind specifically to F-actin filaments with high affinity (1). Fluorescently labeled phallotoxins are very useful tool for investigating the distribution of F-actin. Labeled phallotoxins have similar affinity for both large and small filaments, binding in a stoichiometric ratio of about one phalloidin molecule per actin subunit in muscle and non-muscle cells from various species of plants and animals. Phallotoxins shift the F-actin monomer/polymer equilibrium toward the polymer, lowering the critical concentration for polymerization up to 30-fold (3,4). Phallotoxins also stabilize F-actin filaments, inhibiting depolymerization by cytochalasins, potassium iodide and elevated temperatures. Moreover, phallotoxin-labeled actin filaments remain functional; labeled glycerinated muscle fibers still contract, and labeled actin filaments still move on solid-phase myosin substrates (5,6). Fluorescent phallotoxins can also be used to quantify the amount of F-actin in cells (7,8). Biotin-XX phalloidin stains F-actin with nanomolar affinity. The long flexible spacer group XX facilitates interaction between biotin and avidin or streptavidin which can be labeled with a fluorophore or an enzyme for visualization of F-actin. Biotin-XX phalloidin can also be used for detecting F-actin by electron microscopy. The content in each vial (100 U) is sufficient to stain ~100 samples with 200 uL staining volume per sample.

  • White lyophilized solid soluble in methanol
  • Store at -20°C
  • C57H85N13O14S2
  • MW: 1240

1. Wieland, T. in Phallotoxins, Springer-Verlag, New York (1986).
2. J Muscle Res Cell Motil 9, 370 (1988).
3. Methods Enzymol 85, 514 (1982).
4. Eur J Biochem 165, 125 (1987).
5. Nature 326, 805 (1987).
6. Proc Natl Acad Sci USA 83, 6272 (1986).
7. Blood 69, 945 (1987).
8. Anal Biochem 200, 199 (1992).
9. J Cell Biol 105, 1473 (1987).
10. Proc Natl Acad Sci USA 77, 980 (1980).
11. Nature 284, 405 (1980).
12. CRC Crit Rev Biochem 5, 185 (1978).
13. J Cell Biol 106, 1229 (1988).
14. J Cell Biol 103, 265a (1986).
15. Eur J Cell Biol 24, 176 (1981).
16. Proc Natl Acad Sci USA 74, 5613 (1977).

MSDS (PDF): MSDS 00028
Protocol (PDF): phalloidin

100 U

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