The PI-Cassette™ is a specialized cell sampling and staining device for the NucleoCounter® NC-3000™ instrument. It contains the fluorophore, Propidium Iodide (PI), immobilized in the interior flow channels.
Approximately 60 µl of cell sample is loaded into the cassette, dissolving the PI and mixing to stain the nuclei of dead cells. PI-Cassettes™ can be used to perform viability and cell counts in conjunction with Solution 10 Lysis Buffer and Reagent Y100, respectively, on mammalian and yeast cells. Cells are subsequently analyzed on the NucleoCounter® NC-3000™.
The PI-Cassette™ is used with the NucleoCounter® NC-3000™.