PMA-Lite LED Photolysis Device



Product Description

PMA-Lite™ LED Photolysis Device is specifically designed for photoactivation of propidium monoazide (PMA) and other similar dyes. It can also function as a general photolysis device to provide continuous illumination to 1.5-2 mL-sized vials in a controlled manner. The device can hold up to 18 vials. Multiple LED lights are positioned to provide even and maximal illumination from both the sides and bottom to all vials. An internal fan is used to reduce the heat generated from the lights to ensure near room temperature photolysis. The device has a timer that can be set for 10, 15, 20 or 30 minutes of continuous photolysis. The unit has 120/240V internal converter. A universal outlet adapter is included for customers outside of North America. PMA is a photo-reactive dye that binds to dsDNA with high affinity and can be used for the selective detection of viable pathogens by qPCR. More information can be found on our PMA product pages 40019 and 40013. Receive one free vial of 20 mM PMA (40019) with purchase of the PMA-Lite™ LED Photolysis Device.

PMA-Lite™ LED Photolysis Device. Each device comes with one removable blue tube adapter/rack.

Other PMA-related products: For viability PCR of gram-negative bacteria we highly recommend using our new PMA Enhancer (31038) in conjunction with PMA. PMA Enhancer for Gram Negative Bacteria was designed to improve PMA-mediated discrimination between live and dead gram-negative bacteria. When a sequence from a gram-negative bacteria is amplified by PCR, samples pre-treated with Enhancer show a decrease in the signal from dead cells, with no change in the signal from live cells. Biotium also provides strain-specific PMA Real-Time PCR Bacterial Viability kits with validated primers for 5 selected pathogens: M. tuberculosis, S. aureus, MRSA, Salmonella, and E. coli O157:H7. These kits provide everything that you need for the selective detection of your favorite species of live bacteria by real-time PCR.

1. Nocker, A., Cheung, C.Y., and Kamper, A.K. (2006). Comparison of propidium monoazide with ethidium monoazide for differentiation of live vs. dead bacteria by selective removal of DNA from dead cells. J. Microbio Meth. 67(2), 310-320.
2. Front. Microbiol. (2015), doi:10.3389/fmicb.2015.00295

Download the full PMA Reference List.

Flyer (PDF): FL-PMA

Protocol (PDF): PI-E90002

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life