The Reagent Y100 is used to disrupt the plasma membranes of the yeast cells, rendering the nuclei susceptible to staining with propidium iodide (PI).
It is recommended to use 1:10 volumes of sample and Reagent Y100 (e.g. 50 ml of sample and 450 ml of Reagent Y100).
If the sample media is a coloured solution, such as beer or wine, a dilution prior to the use of Reagent Y100 may be necessary to avoid the colour affecting the fluorescence intensity detected from the cells. Please contact ChemoMetec for further information.
Used to determine the total amount of yeast cells in a sample using the YC-100™ and NC-3000™.
Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.
Expedited shipment may be requested at time of checkout.
Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.
More information about product handling and shelf life