Resazurin, Sodium Salt



Product Description

Resazurin, which is the N-oxide of the fluorescent dye resorufin, is useful for detecting reductive activities in cells and has been widely used for measuring cell proliferation (1,2) and mitochondrial metabolic activity. Resazurin itself is non-fluorescent until it is reduced to the highly red fluorescent resorufin (λExEm: 563/587 nm).

Usually, NADPH or NADH is the reductant that converts resazurin to resorufin in the presence of diaphorase as the enzyme. Thus, resazurin can be used to detect NADH, NADPH, or diaphorase levels. Furthermore, the resazurin/diaphorase/ NADPH system can be used to detect any biochemical or enzyme activity that is involved in a biochemical reaction generating NADH or NADPH (3-9).

Although resazurin is available from most of the chemical suppliers, a trace amount of resorufin contaminant often makes the material unsuitable for bioassay applications. We supply a high grade resazurin that has a minimal background fluorescence. Please also see our ready-to-use Resazurin Cell Viability Assay Kit (30025).

  • λExEm (pH 9) = 604 nm/none
  • Dark solid soluble in DMSO or water
  • Store at 4°C and protect from light
  • C12H6NNaO4
  • MW: 251.17

1. J Ocular Pharmacol Therapeut 26, 1 (2010).
2. J Clin Lab Anal 9, 89 (1995).
3. J Immunol Methods 175, 181 (1994).
4. J Anal Toxicol 8, 273 (1984).
5. Clin Chem 29, 171 (1983).
6. Clin Chim Acta 107, 149 (1980).
7. Clin Chem 26, 61 (1980).
8. Biochem Biophys Acta 484, 249 (1977).

MSDS (PDF): MSDS 10054

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life