In non-apoptotic cells JC-1 will enter the mitochondria which causes a red-shift in fluorescence from green (~529 nm) to red (~590 nm). Depolirazation of the mitochondria will cause a green shift in cells indicating they are apoptotic.
Used for measurements when using the NC-3000™ for the Mitochondrial Potential Assay in conjunction with Solution 8.
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