Firefly luciferase is widely used as a reporter for studying gene regulation and function, and for pharmaceutical screening. It is a very sensitive genetic reporter due to the lack of endogenous activity in mammalian cells or tissues. However, the light production resulting from the reaction leads to formation of suicidal adenyl-oxyluciferin at the enzyme surface. It results in very short half-life of the light emission with a flash-type kinetics. Because of this, flash-type luminescence must be detected immediately after initiating the reaction, which is not compatible with batch analysis of samples.
Biotium’s Steady-Luc™ HTS assay system is a proprietary mixture of substances that modify the enzymatic reaction to produce a long lasting signal (steady glow) by preventing the formation of adenyl-oxyluciferin at the enzyme surface. It is a homogeneous high sensitivity firefly luciferase reporter gene assay kit with a half-life of about 3 hours for the quantification of firefly luciferase expression in mammalian cells, allowing batch processing in microplates.
Glow-type luciferase assays like Steady-Luc™ have lower luminescence signal compared to flash-type assays. The sensitivity and limit of detection of the assay will depend on luciferase expression levels in your experimental system as well as luminometer sensitivity.
The Steady-Luc™ Firefly HTS Assay Kit (Lyophilized) is comparable to our original Steady-Luc™ HTS Assay (catalog no. 30028), but with assay buffer provided in lyophilized format for economical shipping and convenient storage. Simply dissolve the lyophilized assay buffer in the supplied reconstitution buffer before use.
- Steady-Luc Firefly Assay Buffer (Lyophilized)
- Reconstitution Buffer
- Simple: single step homogeneous assay.
- Glow kinetics: luminescence half life of about 3 hours.
- Convenient: lyophilized assay buffer for convenient shipping and storage.
- Economic: save up to 45% compared to other brands.