Ultrafiltration Vial, MWCO=3K



Product Description

Centrifugal ultrafiltration devices with 3K molecular weight cut-off (MWCO) membrane allow molecules with molecular weight smaller than 3,000 to pass through the membrane into the filtrate collection tube, while molecules larger than 3,000 molecular weight are retained on top of the ultrafiltration membrane. Ultrafiltration can be used to rapidly and easily remove small molecules such as Tris, glycerol and glycine from protein solutions, or to concentrate proteins prior to conjugation. They also can be used to remove free dye or free biotin from proteins after conjugation.

For ultrafiltration of proteins, it is recommended to use a MWCO 3- to 6-times smaller than the protein to be retained. Ultrafiltration vials with with MWCO=3K are recommended for ultrafiltration of proteins larger than 10 kDa. Biotium also offers vials with MWCO=10K (catalog no. 22004) for ultrafiltration of antibodies and other proteins larger than 30 kDa. Ultrafiltration is not effective for fractionating proteins (separating one protein from another protein based on size) unless there is at least a 10-fold difference in the protein molecular weights.


Protocol (PDF): PI-Ultrafiltration Vials

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life