The Via1-Cassette™ is a specialized cell sampling and staining device for NucleoCounter® instruments. It contains two immobilized fluorophores, acridine orange (AO) and DAPI.
Approximately 60 µl of cell sample is loaded into the cassette, dissolving the AO and DAPI in the mixing channels. AO stains all cells in the sample, both live and dead cells, thus providing a total count; DAPI stains the dead cells.
Each cassette carries an individual dot-code indicating the exact volume of its counting chamber. This dot-code is read by the NucleoCounter® instrument at time of analysis and is used by the software to calculate cell concentration.
The total volume of analysis within the counting chamber is approximately 1.4 µl, measured during production. Each Via1-Cassette™ has a dot code, read by the NucleoCounter® to accurately calculate cell concentration of each sample.
via-1 cassette via 1