ViaFluor 568-SE
Cell Proliferation Kit



Product Description

ViaFluor™-SE Cell Proliferation Dyes are fluorescent tracers that diffuse passively into cells and covalently label intracellular proteins, resulting in long term cell labeling. They are initially non-fluorescent, but are converted to amine-reactive fluorescent dyes after hydrolysis by intracellular esterases. The dyes then covalently react with amine groups on intracellular proteins, forming fluorescent conjugates that are retained in the cell, while excess unreacted dye is washed away.  Immediately after staining, a single, bright fluorescent population will be detected by flow cytometry.

With each cell division, daughter cells inherit roughly half of the fluorescent label, allowing the number of cell divisions that occur after labeling to be detected by the appearance of successively dimmer fluorescent peaks on a flow cytometry histogram compared to cells analyzed immediately after staining (Figs. 1 and 2).

Cell proliferation dyes can be used to track cell divisions in vivo or in vitro. The staining also can withstand fixation and permeabilization for subsequent immunostaining. ViaFluor™568-SE Cell Proliferation Dye has excitation/emission at  570/606 nm (Figure 3) for detection in the PE-Texas Red® channel with blue laser excitation, or detection in the Texas Red® channel with green laser excitation.

Each kit contains 10 lyophilized dye vials, anhydrous DMSO for preparing stock solutions, and a labeling protocol. One kit can be used to label at least 2×108 cells; the final number of assays that can be performed per kit depends on the dye concentration used (see product protocol under downloads). Biotium also offers blue fluorescent ViaFluor™405-SE and green fluorescent ViaFluor™CFSE Cell Proliferation Kits. [one_half padding=”0 10px 0 0″]

ViaFluor diagram red
Figure 1. Principal of cell division tracking by flow cytometry using ViaFluor™ dyes.

[/one_half][one_half_last padding=”0 0px 0 10px”]

ViaFluor 568
Figure 2. Cell division tracking with ViaFluor™ 568-SE in Jurkat cells. Cells were labeled with ViaFluor™ 568-SE Cell Proliferation Dye, then maintained in culture for various lengths of time to allow cell division. From right to left: 0 days (darkest peak), 1 day, 2 days, or 3 days (lightest peak). Unstained cells are shown in gray. Fluorescence was detected in the PE-Texas Red channel of a BD LSRII flow cytometer with 488 nm excitation.


ViaFluor 568 spectra
Figure 3. Absorption and emission spectra of ViaFluor™ 568-SE.

Texas Red is a registered trademark of Life Technologies.


Flyer (PDF): Apoptosis, Necrosis, and Cell Viability Assays
MSDS (PDF): MSDS 30080
PI-30050, 30068, 30080

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life