WarmStart Enzyme Modification Kit


Product Description

This is a special order product. The WarmStart™ Enzyme Modification Kit allows temperature control of enzyme activity to be applied to non-thermostable enzymes like reverse transcriptase. Similar to our HotStart polymerase modification reagent (catalog no. 29054), the enzyme is inactivated by modification of lysine residues with a novel chemical modifier. While HotStart modification is reversed by heating to 90°C or higher, WarmStart™ protein activity is restored by heating to 45°C or higher. Potential applications include modification of:

  • Reverse transcriptase
  • Bst DNA polymerase
  • E. coli DNA polymerase I
  • Restriction enzymes
  • Nucleases
  • Proteases

The trial size WarmStart™ modification kit (cat. no. 29053-T) contains reagents for modifying up to 0.1 mg protein. The full size kit (cat. no. 29053) contains reagents for modifying up to 0.5 mg protein.


Flyer (PDF): Genomics & Proteomics

Protocol (PDF): PI-29053

Shipment Method: Shipping and handling methods will be assessed and calculated at time of shipment based upon item(s) storage temperature conditions.

Expedited shipment may be requested at time of checkout.

Please note that products will be shipped at ambient temperature. This will not affect performance if the products are kept at the indicated temperature for long term storage. Please refer to the package insert for correct storage conditions.

More information about product handling and shelf life