X-gal (5-Bromo-4-chloro-3-indolyl-β-galactopyranoside) is the most widely used chromogenic substrate for β-galactosidase. Enzyme hydrolysis of the substrate yields a dark blue colored precipitate (maximum absorbance 615 nm).
- White solid soluble in DMSO
- Store desiccated at -20°C and protect from light
- MW: 408.6
1. Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, B. 14, p 186 (1989).
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